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It depends. If you're sending the email through the Outlook or Gmail Sales Insight plugins then unsubscribe is not respected since that is a true 1:1 communication (and also not sending from Marketo servers). If you are sending from Sales Insight in SFDC or MS Dynamics, it depends on what the settings are in Admin and how the email is built. The different ways are detailed here Configure Unsubscribe Footers in Marketo Sales Insight - Marketo Docs - Product Documentation . You can have the emails either ignore or respect unsubscribe settings dependent on if the email uses a template, does not use a template, or is operational.