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The easiest way to ensure this is done correctly is to just duplicate the emails so there are 4 in total, each with their own subject line/copy combination. Then create a smart campaign and have a flow step like this:
1. Send Email
Choice 1: if random sample is 25%: send Email 1
Choice 2: if random sample is 25%: send Email 2
Choice 3: if random sample is 25%: send Email 3
Choice 4: if random sample is 25%: send Email 4
Default Choice: do nothing
This ensures every person in the list is only sent one variation of the email and each email is sent to about the same size of the list (25% of 160,000).
Thanks, Devraj. I was trying to keep it in an email program, but your route seems like a cleaner option.
It's much easier for reporting purposes as well just to have each email variation created as a separate email then you don't need to mess with the test variant constraints.
That's very true. Thanks, Devraj!