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Marketo will de-duplicate based on email address. So if you fill out a form with the same email address, Marketo will interpret it as the same person and will update the existing lead on the second form fill.
It is not exactly a merge taking place because the second lead was never actually created; Marketo just updated the first.
You couldn't prevent the lead from being updated. However you could look at whatever rules are moving leads out of of your test queue and adjust them, so that leads declared invalid stay there regardless of any additional activity. I think that would solve your root requirement.